光质光强对青钱柳叶黄酮类化合物积累的影响

摘□要

本论文以青钱柳当年生苗为对象，研究了不同光质和光强处理对青钱柳叶中总黄酮及各黄酮类化合物单体含量的影响，研究结果如下：

（1）光质对青钱柳叶中总黄酮积累影响差异十分显著。同光强条件下，LED蓝光、LED绿光、LED红光条件下总黄酮积累量都较大。所有光照强度条件下，LED紫光处理的青钱柳叶中总黄酮含量都最少。不同光质对总黄酮积累能力大小依次是：蓝gt;绿gt;红gt;白gt;紫。光强对总黄酮积累能力大小依次是高gt;低gt;中。青钱柳叶中总黄酮的含量随处理时间增加大体呈先上升后下降趋势。用高光强蓝光或绿光处理60d时青钱柳叶中总黄酮含量最大。

（2）青钱柳叶中的槲皮素含量在中光照强度LED蓝光、高光照强度LED蓝光及低光照强度LED红光条件处理的含量最大。不同光质对槲皮素积累能力大小依次是：蓝gt;红gt;绿gt;白gt;紫。不同光强对槲皮素积累能力大小依次是高光强gt;中光强gt;低光强。高光照强度LED蓝光条件最有利于槲皮素积累。低光照强度LED紫光条件有含量较少的。

（3）光质对青钱柳叶中山奈酚积累影响差异显著，光强对其影响不显著。山奈酚在中光强LED蓝光及高光强LED绿光条件下含量最大。同一光强条件下，LED绿光对山奈酚积累的作用与LED蓝光条件相近。不同光质对山奈酚积累能力大小依次是：蓝gt;绿gt;红gt;白gt;紫。光照强度宜选择中度或高度光强。含量较少的条件有低光强紫光。

（4）光质对青钱柳叶中异槲皮苷积累影响差异显著，光质光强有交互作用。高光强LED蓝光条件下异槲皮苷积累最大且远大于其它光质光强条件。同光强条件下，LED红光和LED蓝光条件最有利于异槲皮苷的积累。不同光质对异槲皮苷积累能力大小依次是：蓝gt;红gt;绿gt;紫gt;白。光强对于其积累能力规律大致为：高gt;低gt;中。从均值来看，LED紫光对异槲皮苷的积累能力大于LED白光。

关键词：青钱柳；黄酮；异槲皮苷；光质；光强

The Effect of Light Quality and Intensity on the Accumulation of Flavonoids in Cyclocarya paliurus Leaves

ABSTRACT

In this paper, Cyclocarya paliurus under five different light quality and three different light intensity conditions treated as the main object of study, the content of total flavonoid three kinds of flavonoids monomer was measured and analyzed. The results are followed:

(1) The effect of light quality on total flavonoids accumulation in Cyclocarya paliurus is very significant. When light intensity is under the same condition, accumulation of total flavonoids under the conditions of blue, green, red light quality are stronger. Accumulation of total flavonoids are less strong under the condition of purple light quality. The descending ability of different light quality to accumulate total flavonoids are: bluegt;greengt;redgt;whitegt; purple. The descending ability of different light intensity to accumulate total flavonoids are followed by highgt; lowgt;medium. Total Flavonoids should be at maximum under the condition of high light intensity blue or green light quality for 60 days.

(2) The content of quercetin is at the maximum under the condition of blue light quality high intensity and red light quality low light intensity. The descending ability of different light quality to accumulate quercetin are: bluegt;redgt;greengt;whitegt;purple. The descending ability of different light intensity to accumulate quercetin is followed by high light intensitygt; light intensitygt; Low light intensity. High intensity blue light condition is the most suitable for the accumulation of quercetin. Low light intensity violet light condition accumulates less quercetin.

(3) The impact of light quality on kaempferol accumulation in Cyclocarya paliurus is very significant, while the light intensity has no significant effect. Kaempferol accumulation in blue light high intensity and green light high intensity conditions is at the maximum. When light intensity is under the same condition, the green light quality and the blue light quality have similar effect to kaempferol accumulation. The descending ability of different light quality to accumulate kaempferol are: bluegt;greengt;redgt;whitegt;purple. We should choose moderate or high intensity. Low light intensity violet light condition accumulates less kaempferol.

(4) The impact of light quality on Isoquercitrin accumulation in Cyclocarya paliurus is very significant, and there is a strong interaction between light quality and light intensity. Isoquercitrin accumulation is much more than the others under the condition of high intensity blue light quality. When light intensity is under the same conditions, the red and blue light quality are the most favorable to the accumulation of Isoquercitrin. The descending ability of different light quality to accumulate Isoquercitrin are:bluegt;redgt;greengt;Purplegt;White. Light intensity for their ability to accumulate Isoquercitrin generally are: Highgt;Lowgt;medium. In short, the ability of purple light quality to accumulate Isoquercitrin is greater than white light quality.

Key words: Cyclocarya paliurus; flavones; Isoquercetin; light quality; light intensity